Hotspot

psbK-psbI

LSC · 6900-7348 · 449 bp

Back to Atriplex

Pi 0.0237

MarkerSeek score 66.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 19
pi: 0.0237
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCGCGTCCAGGATTAC	50.0	50.0	57.9	57.7	586-591 bp	1.0	80.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTCTTCGCGTCCAGGATTAC	45.0	50.0	58.3	57.7	583-588 bp	1.0	78.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTCTTCGCGTCCAGGATTAC	45.0	50.0	58.3	57.7	584-589 bp	1.0	78.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTCTTCGCGTCCAGGATTAC	45.0	50.0	58.3	57.7	585-590 bp	1.0	78.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	573-578 bp	1.0	75.9