Hotspot

rps15

SSC · 153526-153789 · 264 bp

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Pi 0.00722

MarkerSeek score 29.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 5
pi: 0.00722
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GTAGAAGAAGGGCGTCTGTG	TGGATAAATAGCTGCGCACA	55.0	45.0	58.0	57.7	1007-1052 bp	1.0	82.2
rps15_p2	GCCAGTGACAGCTTCGAATA	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1058-1103 bp	1.0	82.0
rps15_p3	ACTAATAAGGCCCACCCCTT	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1238-1283 bp	1.0	81.3
rps15_p4	TAAGGTAAATCGGCAGCGAC	TGGATAAATAGCTGCGCACA	50.0	45.0	58.1	57.7	1114-1159 bp	1.0	81.3
rps15_p5	ACCCCTTCGACTCGTTCTAA	TGGATAAATAGCTGCGCACA	50.0	45.0	58.1	57.7	1225-1270 bp	1.0	80.7