Hotspot

ndhE-ndhG

SSC · 148338-148579 · 242 bp

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Pi 0.0196

MarkerSeek score 54.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 242
variable_sites: 10
pi: 0.0196
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACAAGTACATGTTCGAGCATCA	TAGTTGCTTTGATCGGTGCA	40.9	45.0	58.1	57.8	297-306 bp	1.0	67.1
ndhE-ndhG_p2	ACAAGTACATGTTCGAGCATCA	TGCTTTGATCGGTGCAATTG	40.9	45.0	58.1	57.6	293-302 bp	1.0	65.8
ndhE-ndhG_p3	ACAAGTACATGTTCGAGCATCA	GTTGCTTTGATCGGTGCAAT	40.9	45.0	58.1	57.6	295-304 bp	1.0	65.7
ndhE-ndhG_p4	ACAAGTACATGTTCGAGCATCA	CTTTGATCGGTGCAATTGCC	40.9	50.0	58.1	58.4	291-300 bp	1.0	65.7
ndhE-ndhG_p5	GGCACTCAAAACAAGTACATGT	TAGTTGCTTTGATCGGTGCA	40.9	45.0	57.4	57.8	307-316 bp	1.0	63.0