Hotspot

rpl32-trnL-UAG

SSC · 143770-144514 · 745 bp

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Pi 0.00908

MarkerSeek score 43.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 745
variable_sites: 18
pi: 0.00908
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAAAGGGGGAGTCTCTGA	GAGCGGGGACTAAGATTGTG	50.0	55.0	57.3	58.1	1717-1724 bp	1.0	74.9
rpl32-trnL-UAG_p2	AGAAAAGGGGGAGTCTCTGA	TTGAAGAGCGGGGACTAAGA	50.0	50.0	57.3	58.1	1722-1729 bp	1.0	74.8
rpl32-trnL-UAG_p3	AGAAAAGGGGGAGTCTCTGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.3	57.9	1150-1156 bp	1.0	74.8
rpl32-trnL-UAG_p4	AGAAAAGGGGGAGTCTCTGA	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.3	58.1	1670-1677 bp	1.0	74.6
rpl32-trnL-UAG_p5	AGAAAAGGGGGAGTCTCTGA	TGGGTAAAAATAGCACCGGG	50.0	50.0	57.3	57.9	1655-1662 bp	1.0	74.3