Hotspot

ndhF-rpl32

SSC · 142527-143595 · 1069 bp

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Pi 0.0065

MarkerSeek score 47.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1069
variable_sites: 20
pi: 0.0065
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCCTGCTCCGATTAACAACG	GCTTTTTGGAAATTGAAGTACGC	50.0	39.1	58.0	57.7	1180-1187 bp	1.0	55.6
ndhF-rpl32_p2	AGAAGAAGTCCTGCTCCGAT	GCTTTTTGGAAATTGAAGTACGC	50.0	39.1	58.1	57.7	1188-1195 bp	1.0	54.5
ndhF-rpl32_p3	TCCTGCTCCGATTAACAACG	CGCTTTTTGGAAATTGAAGTACG	50.0	39.1	58.0	57.5	1181-1188 bp	1.0	53.8
ndhF-rpl32_p4	AAGAAGAAGTCCTGCTCCGA	GCTTTTTGGAAATTGAAGTACGC	50.0	39.1	58.4	57.7	1189-1196 bp	1.0	52.7
ndhF-rpl32_p5	AGAAGAAGTCCTGCTCCGAT	CGCTTTTTGGAAATTGAAGTACG	50.0	39.1	58.1	57.5	1189-1196 bp	1.0	52.7