Hotspot

petA-psbJ

LSC · 87647-88574 · 928 bp

Back to Atraphaxis

Pi 0.0107

MarkerSeek score 52.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 22
pi: 0.0107
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTCGAGAAGGTTCA	GGGTCATATTCCGGATTGGG	40.9	55.0	57.6	57.8	1005-1009 bp	1.0	61.0
petA-psbJ_p2	AGAAACAGTTCGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.6	57.6	998-1002 bp	1.0	59.7
petA-psbJ_p3	AGAAACAGTTCGAGAAGGTTCA	AACCGGGATTGTTGTGATCG	40.9	50.0	57.6	58.6	1048-1052 bp	1.0	58.1
petA-psbJ_p4	AGAAACAGTTCGAGAAGGTTCA	CGGGATTGTTGTGATCGGTT	40.9	50.0	57.6	58.6	1045-1049 bp	1.0	58.1
petA-psbJ_p5	GAAACAGTTCGAGAAGGTTCA	GGGTCATATTCCGGATTGGG	42.9	55.0	56.1	57.8	1004-1008 bp	1.0	57.4