Hotspot

rbcL-accD

LSC · 81127-81558 · 432 bp

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Pi 0.00728

MarkerSeek score 50.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 8
pi: 0.00728
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCCAAATGGAGTCCTGAAC	TGCCCCCTATTTGCATGAAA	50.0	45.0	58.1	57.8	591-593 bp	1.0	78.7
rbcL-accD_p2	TGCCAAATGGAGTCCTGAAC	TTGCCCCCTATTTGCATGAA	50.0	45.0	58.1	57.8	592-594 bp	1.0	78.7
rbcL-accD_p3	CTGCCAAATGGAGTCCTGAA	TGCCCCCTATTTGCATGAAA	50.0	45.0	57.8	57.8	592-594 bp	1.0	77.8
rbcL-accD_p4	CTGCCAAATGGAGTCCTGAA	TTGCCCCCTATTTGCATGAA	50.0	45.0	57.8	57.8	593-595 bp	1.0	77.8
rbcL-accD_p5	GCCAAATGGAGTCCTGAACT	TGCCCCCTATTTGCATGAAA	50.0	45.0	57.8	57.8	590-592 bp	1.0	77.7