Hotspot

atpB-rbcL

LSC · 78851-79674 · 824 bp

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Pi 0.0014

MarkerSeek score 54.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 3
pi: 0.0014
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTTGCCTGGTGGAAAAGCTA	GCTTTGAATCCAACACCTGC	50.0	50.0	58.1	57.9	991-1023 bp	1.0	80.7
atpB-rbcL_p2	AACCCCAGGACTAGACGTAG	GCTTTGAATCCAACACCTGC	55.0	50.0	57.9	57.9	910-942 bp	1.0	80.5
atpB-rbcL_p3	CTTGCCTGGTGGAAAAGCTA	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	955-987 bp	1.0	80.4
atpB-rbcL_p4	GCCTGGTGGAAAAGCTACAT	GCTTTGAATCCAACACCTGC	50.0	50.0	58.2	57.9	988-1020 bp	1.0	80.2
atpB-rbcL_p5	CCAATGATTTGAGCGACACG	GCTTTGAATCCAACACCTGC	50.0	50.0	57.8	57.9	957-989 bp	1.0	80.2