Hotspot

rps4-trnT-UGU

LSC · 70382-71105 · 724 bp

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Pi 0.0067

MarkerSeek score 63.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 12
pi: 0.0067
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGTCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	804-845 bp	1.0	82.3
rps4-trnT-UGU_p2	CCCCCAGACGTCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	773-814 bp	1.0	82.3
rps4-trnT-UGU_p3	CCCCCAGACGTCGTATTTTT	CTTTTATGGGAGCCCGCTTA	50.0	50.0	57.9	57.7	825-866 bp	1.0	80.6
rps4-trnT-UGU_p4	CCCCCAGACGTCGTATTTTT	TTTTATGGGAGCCCGCTTAG	50.0	50.0	57.9	57.7	824-865 bp	1.0	80.6
rps4-trnT-UGU_p5	TAAAACCCCCAGACGTCGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	809-850 bp	1.0	80.2