Hotspot

rpoB-trnC-GCA

LSC · 48670-50106 · 1437 bp

Back to Atraphaxis

Pi 0.0028

MarkerSeek score 52.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1437
variable_sites: 10
pi: 0.0028
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CTTACCGCTCGGCCATGT	50.0	61.1	57.3	59.8	1442-1498 bp	1.0	48.4
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	TTACCGCTCGGCCATGTC	50.0	61.1	57.3	59.8	1441-1497 bp	1.0	48.4
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	CTTACCGCTCGGCCATGTC	50.0	63.2	57.3	60.9	1442-1498 bp	1.0	47.8
rpoB-trnC-GCA_p4	ACATTCCCTCATTTCCATCCC	CTTACCGCTCGGCCATGT	47.6	61.1	57.7	59.8	1444-1500 bp	1.0	44.2
rpoB-trnC-GCA_p5	ACATTCCCTCATTTCCATCCC	TTACCGCTCGGCCATGTC	47.6	61.1	57.7	59.8	1443-1499 bp	1.0	44.2