Hotspot

trnR-UCU-atpA

LSC · 32043-32195 · 153 bp

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Pi 0.0322

MarkerSeek score 62.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 8
pi: 0.0322
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAGAAGCGTCCATTGTCT	ACCAAGACACTCACTGAGGA	45.0	50.0	56.9	57.9	316-321 bp	1.0	73.8
trnR-UCU-atpA_p2	AATGAGAAGCGTCCATTGTCT	ACCAAGACACTCACTGAGGA	42.9	50.0	57.7	57.9	317-322 bp	1.0	71.6
trnR-UCU-atpA_p3	ATGGTAATGAGAAGCGTCCA	ACCAAGACACTCACTGAGGA	45.0	50.0	56.6	57.9	322-327 bp	1.0	71.3
trnR-UCU-atpA_p4	TGGTAATGAGAAGCGTCCAT	ACCAAGACACTCACTGAGGA	45.0	50.0	56.6	57.9	321-326 bp	1.0	71.3
trnR-UCU-atpA_p5	TGGTAATGAGAAGCGTCCATT	ACCAAGACACTCACTGAGGA	42.9	50.0	57.4	57.9	321-326 bp	1.0	69.2