Hotspot

petB

LSC · 74770-76446 · 1677 bp

Back to Atractylodes

Pi 0.00107

MarkerSeek score 51.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1677
variable_sites: 4
pi: 0.00107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	ACGGTTCTAGATCTGGTCCA	ATCGTTCCTACCCCAAGTGA	50.0	50.0	57.5	58.1	1800 bp	1.0	75.5
petB_p2	ACGGTTCTAGATCTGGTCCA	TCGTTCCTACCCCAAGTGAT	50.0	50.0	57.5	58.1	1799 bp	1.0	75.5
petB_p3	AGACGAACTACTGTAGGGGT	ATCGTTCCTACCCCAAGTGA	50.0	50.0	57.1	58.1	1780 bp	1.0	72.6
petB_p4	AGACGAACTACTGTAGGGGT	TCGTTCCTACCCCAAGTGAT	50.0	50.0	57.1	58.1	1779 bp	1.0	72.6
petB_p5	GATCTGGTCCAAGACGAACT	TCGTTCCTACCCCAAGTGAT	50.0	50.0	57.0	58.1	1790 bp	1.0	71.4