Hotspot

ndhF

SSC · 125855-128089 · 2235 bp

Back to Atractylodes

Pi 0.000179

MarkerSeek score 50.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 1
pi: 0.000179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGTAAGATCCGGTGAATCG	TCAATGCAACAGCAAGATGC	52.4	45.0	58.0	57.6	2372-2395 bp	1.0	69.8
ndhF_p2	AGGTAAGATCCGGTGAATCG	TCAATGCAACAGCAAGATGC	50.0	45.0	56.8	57.6	2371-2394 bp	1.0	68.5
ndhF_p3	CGAAAGAGGTAAGATCCGGTG	TCAATGCAACAGCAAGATGC	52.4	45.0	58.2	57.6	2377-2400 bp	1.0	68.4
ndhF_p4	CGAAAGAGGTAAGATCCGGT	TCAATGCAACAGCAAGATGC	50.0	45.0	56.7	57.6	2377-2400 bp	1.0	67.9
ndhF_p5	TCGAAAGAGGTAAGATCCGGT	TCAATGCAACAGCAAGATGC	47.6	45.0	58.3	57.6	2378-2401 bp	1.0	67.8