Hotspot

trnL-TAG-rpl32

SSC · 123765-124633 · 869 bp

Back to Atractylodes

Pi 0.00207
MarkerSeek score 52.80
Primer available yes
Label trnL-TAG-rpl32

Feature Metadata

label_name
trnL-TAG-rpl32
feature_type
igs
region
SSC
length_bp
869
variable_sites
4
pi
0.00207
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 1001 bp 1.0 77.3
trnL-TAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 1002 bp 1.0 77.3
trnL-TAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 984 bp 1.0 77.1
trnL-TAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 985 bp 1.0 77.1
trnL-TAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAGGAAAGGATATTGGGCGG 50.0 50.0 57.9 57.6 1011 bp 1.0 76.4