Hotspot

atpB-rbcL

LSC · 54516-55244 · 729 bp

Back to Atractylodes

Pi 0.00247

MarkerSeek score 62.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 4
pi: 0.00247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	899-923 bp	1.0	72.9
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	898-922 bp	1.0	72.6
atpB-rbcL_p3	CTGGCGGAAAAGCTACATCT	GTGGTGACATAAATCCCTCCC	50.0	52.4	58.0	58.1	860-884 bp	1.0	72.5
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	826-850 bp	1.0	72.5
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	825-849 bp	1.0	72.2