Hotspot

trnT-TGT-trnF-GAA

LSC · 46482-47918 · 1437 bp

Back to Atractylodes

Pi 0.00269

MarkerSeek score 61.60

Primer available yes

Label trnT-TGT-trnF-GAA

Feature Metadata

label_name: trnT-TGT-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1437
variable_sites: 9
pi: 0.00269
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnF-GAA_p1	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1524-1545 bp	1.0	79.6
trnT-TGT-trnF-GAA_p2	GGAATCGAACCGATGACCAT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.8	58.1	1556-1577 bp	1.0	78.7
trnT-TGT-trnF-GAA_p3	ATGCGATGCTCTAACCTCTG	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	1526-1547 bp	1.0	76.4
trnT-TGT-trnF-GAA_p4	TGCGATGCTCTAACCTCTGA	TTTGAACTGGTGACACGAGG	50.0	50.0	58.5	58.1	1525-1546 bp	1.0	76.4
trnT-TGT-trnF-GAA_p5	GCGATGCTCTAACCTCTGAG	TACCAACTGAGCTATCCCGA	55.0	50.0	57.9	57.6	1486-1507 bp	1.0	76.4