Hotspot

rps4-trnT-TGT

LSC · 46031-46408 · 378 bp

Back to Atractylodes

Pi 0

MarkerSeek score 50.50

Primer available yes

Label rps4-trnT-TGT

Feature Metadata

label_name: rps4-trnT-TGT
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-TGT_p1	CCAGCTCTGGGCCTTTTATT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	494-527 bp	1.0	81.7
rps4-trnT-TGT_p2	CCAGCTCTGGGCCTTTTATT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.9	57.8	462-495 bp	1.0	80.9
rps4-trnT-TGT_p3	CCAGCTCTGGGCCTTTTATT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	57.9	57.5	492-525 bp	1.0	78.6
rps4-trnT-TGT_p4	CCAGCTCTGGGCCTTTTATT	TCAGAGGTTAGAGCATCGCA	50.0	50.0	57.9	58.5	493-526 bp	1.0	78.6
rps4-trnT-TGT_p5	TTAGTTAGCCCCGGTAAAGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.3	57.9	476-509 bp	1.0	77.4