Hotspot

matK-rps16

LSC · 3578-5194 · 1617 bp

Back to Atractylodes

Pi 0.000989

MarkerSeek score 45.50

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1617
variable_sites: 4
pi: 0.000989
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TCAAGAAGGGCTCCAGAAGA	CCCAGAACAAGGAAACACCA	50.0	50.0	58.0	57.9	2942-2945 bp	1.0	79.6
matK-rps16_p2	AGAAGGGCTCCAGAAGATGT	CCCAGAACAAGGAAACACCA	50.0	50.0	58.0	57.9	2939-2942 bp	1.0	79.5
matK-rps16_p3	TCAAGAAGGGCTCCAGAAGA	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.0	57.9	2644-2647 bp	1.0	79.5
matK-rps16_p4	AGAAGGGCTCCAGAAGATGT	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.0	57.9	2641-2644 bp	1.0	79.4
matK-rps16_p5	TCAAGAAGGGCTCCAGAAGA	AGCCGTCTATCGAATCGTTG	50.0	50.0	58.0	57.9	2573-2574 bp	1.0	79.1