Hotspot

trnH-GTG-psbA

LSC · 78-469 · 392 bp

Back to Atractylodes

Pi 0.00408
MarkerSeek score 50.00
Primer available yes
Label trnH-GTG-psbA

Feature Metadata

label_name
trnH-GTG-psbA
feature_type
igs
region
LSC
length_bp
392
variable_sites
4
pi
0.00408
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GTG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 510-517 bp 1.0 76.9
trnH-GTG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 524-531 bp 1.0 76.9
trnH-GTG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 523-530 bp 1.0 76.8
trnH-GTG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 525-532 bp 1.0 76.8
trnH-GTG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 511-518 bp 1.0 76.4