Hotspot

trnL-UAA

LSC · 47109-47158 · 50 bp

Back to Atractylis

Pi 0.02
MarkerSeek score 61.60
Primer available yes
Label trnL-UAA

Feature Metadata

label_name
trnL-UAA
feature_type
tRNA
region
LSC
length_bp
50
variable_sites
1
pi
0.02
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA_p1 ACATGTCAATACCGGCAACA GGAATTTTGAACCGCTAACGA 45.0 42.9 57.8 57.2 171 bp 1.0 64.4
trnL-UAA_p2 ACATGTCAATACCGGCAACA AGGAATTTTGAACCGCTAACG 45.0 42.9 57.8 57.2 172 bp 1.0 64.3
trnL-UAA_p3 ACATGTCAATACCGGCAACA GGAATTTTGAACCGCTAACGAA 45.0 40.9 57.8 57.9 171 bp 1.0 61.7
trnL-UAA_p4 ACATGTCAATACCGGCAACA AAGGAATTTTGAACCGCTAACG 45.0 40.9 57.8 57.8 173 bp 1.0 61.7
trnL-UAA_p5 ACATGTCAATACCGGCAACA GGAATTTTGAACCGCTAACG 45.0 45.0 57.8 55.7 171 bp 1.0 60.3