Hotspot

rps16

LSC · 5258-6368 · 1111 bp

Back to Atractylis

Pi 0.0036

MarkerSeek score 51.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1111
variable_sites: 4
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAGCAAGAAAAGAAGGAAA	ACCGACAGAACCAATGACTA	35.0	45.0	52.6	56.2	1279-1280 bp	1.0	40.3
rps16_p2	AGAGCAAGAAAAGAAGGAAA	GTACCGACAGAACCAATGACT	35.0	47.6	52.6	57.7	1281-1282 bp	1.0	40.2
rps16_p3	AGAGCAAGAAAAGAAGGAAAT	GTACCGACAGAACCAATGACT	33.3	47.6	53.3	57.7	1281-1282 bp	1.0	40.2
rps16_p4	AGAGCAAGAAAAGAAGGAAA	TACCGACAGAACCAATGACT	35.0	45.0	52.6	56.2	1280-1281 bp	1.0	40.2
rps16_p5	AGAGCAAGAAAAGAAGGAAA	GTACCGACAGAACCAATGAC	35.0	50.0	52.6	56.2	1281-1282 bp	1.0	40.2