Hotspot

ndhD

SSC · 120861-122363 · 1503 bp

Back to Atractylis

Pi 0.00399

MarkerSeek score 48.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 6
pi: 0.00399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTCTGGTCCAAGTGTATCTT	TTCATTAGCCAACTAACTCTT	40.0	33.3	53.6	52.5	1586 bp	1.0	40.3
ndhD_p2	TTCTGGTCCAAGTGTATCTT	TTTCATTAGCCAACTAACTCT	40.0	33.3	53.6	52.5	1587 bp	1.0	40.3
ndhD_p3	TTTCTGGTCCAAGTGTATCT	TTCATTAGCCAACTAACTCTT	40.0	33.3	53.6	52.5	1587 bp	1.0	40.3
ndhD_p4	TTTCTGGTCCAAGTGTATCT	TTTCATTAGCCAACTAACTCT	40.0	33.3	53.6	52.5	1588 bp	1.0	40.3
ndhD_p5	TTCTGGTCCAAGTGTATCTT	TTTCATTAGCCAACTAACTCTT	40.0	31.8	53.6	53.4	1587 bp	1.0	40.3