Hotspot

accD

LSC · 56907-58520 · 1614 bp

Back to Atractylis

Pi 0.00496

MarkerSeek score 54.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1614
variable_sites: 8
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATGCAAATATGGAGCAAGA	ACAAAGAAAACCCCATTCGTC	38.1	42.9	55.6	57.0	1727-1745 bp	1.0	40.4
accD_p2	TCATGCAAATATGGAGCAAGA	CAACAAAGAAAACCCCATTCGT	38.1	40.9	55.6	57.9	1729-1747 bp	1.0	40.4
accD_p3	TCATGCAAATATGGAGCAAGAA	ACAAAGAAAACCCCATTCGTC	36.4	42.9	56.4	57.0	1727-1745 bp	1.0	40.4
accD_p4	TTCATGCAAATATGGAGCAAGA	ACAAAGAAAACCCCATTCGTC	36.4	42.9	56.4	57.0	1728-1746 bp	1.0	40.4
accD_p5	TCATGCAAATATGGAGCAAGA	ACAAAGAAAACCCCATTCGT	38.1	40.0	55.6	55.8	1727-1745 bp	1.0	40.4