Hotspot

psaC-ndhD

SSC · 120739-120860 · 122 bp

Back to Atractylis

Pi 0.0583

MarkerSeek score 78.10

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 122
variable_sites: 7
pi: 0.0583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	ACAGAGGATTGTGTCGGTTG	AGGAACCCGCAAATATTGGT	50.0	45.0	57.8	57.5	292-294 bp	1.0	76.9
psaC-ndhD_p2	CAGAGGATTGTGTCGGTTGT	AGGAACCCGCAAATATTGGT	50.0	45.0	57.8	57.5	291-293 bp	1.0	76.9
psaC-ndhD_p3	AAGAGATGTGAATCCGCCTG	AGGAACCCGCAAATATTGGT	50.0	45.0	57.7	57.5	271-273 bp	1.0	75.9
psaC-ndhD_p4	CGCAGCATGGGTATAGCTTA	AGGAACCCGCAAATATTGGT	50.0	45.0	57.5	57.5	202-204 bp	1.0	75.7
psaC-ndhD_p5	GAACAGAGGATTGTGTCGGT	AGGAACCCGCAAATATTGGT	50.0	45.0	57.5	57.5	294-296 bp	1.0	74.6