Hotspot

rpl22-rps19

LSC · 83928-84008 · 81 bp

Back to Atractylis

Pi 0.013

MarkerSeek score 64.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 1
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGGAACGTCCACGAATT	TCCGAGGACATGCAAAAAGC	50.0	50.0	58.0	59.1	228-237 bp	1.0	72.7
rpl22-rps19_p2	TTACTCTTCGCGCTTTGTGA	TCCGAGGACATGCAAAAAGC	45.0	50.0	57.9	59.1	201-210 bp	1.0	71.9
rpl22-rps19_p3	CGTAGGAACGTCCACGAATT	AACAGATCGTATGGTAGGCC	50.0	50.0	58.0	56.8	281-290 bp	1.0	71.7
rpl22-rps19_p4	TTACTCTTCGCGCTTTGTGA	AACAGATCGTATGGTAGGCC	45.0	50.0	57.9	56.8	254-263 bp	1.0	70.8
rpl22-rps19_p5	CGTAGGAACGTCCACGAATT	TTCCGAGGACATGCAAAAAG	50.0	45.0	58.0	56.6	229-238 bp	1.0	70.6