Hotspot

trnP-UGG-psaJ

LSC · 66779-67091 · 313 bp

Back to Atractylis

Pi 0.0228
MarkerSeek score 67.70
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
313
variable_sites
7
pi
0.0228
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 397-403 bp 1.0 81.5
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 396-402 bp 1.0 81.5
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 408-414 bp 1.0 81.4
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 407-413 bp 1.0 81.4
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 410-416 bp 1.0 81.3