Hotspot

trnY-GUA-trnE-UUC

LSC · 11718-11793 · 76 bp

Back to Atractylis

Pi 0.0132
MarkerSeek score 60.10
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
76
variable_sites
1
pi
0.0132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CGAATTTACAGTCCGTCCCC CGACTTCTCGCTTTCACTGA 55.0 50.0 58.4 57.9 170 bp 1.0 77.2
trnY-GUA-trnE-UUC_p2 ACGAATTTACAGTCCGTCCC CGACTTCTCGCTTTCACTGA 50.0 50.0 57.6 57.9 171 bp 1.0 77.1
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC CACTGAGACAACGAGGGTTC 55.0 55.0 58.4 58.2 156 bp 1.0 76.0
trnY-GUA-trnE-UUC_p4 CGAATTTACAGTCCGTCCCC ACGACTTCTCGCTTTCACTG 55.0 50.0 58.4 58.2 171 bp 1.0 75.8
trnY-GUA-trnE-UUC_p5 ACGAATTTACAGTCCGTCCC CACTGAGACAACGAGGGTTC 50.0 55.0 57.6 58.2 157 bp 1.0 75.8