Hotspot

ycf1

SSC · 119950-125058 · 5109 bp

Back to Athyrium

Pi 0.0165

MarkerSeek score 47.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5109
variable_sites: 266
pi: 0.0165
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTGAGTTGTTTCAGCGTCC	TGGGCGATCCATTGATGTC	50.0	52.6	57.3	57.6	176 bp	1.0	71.4
ycf1_p2	CTTGAGTTGTTTCAGCGTCC	GGGCGATCCATTGATGTCG	50.0	57.9	57.3	58.8	175 bp	1.0	68.5
ycf1_p3	TTGAGTTGTTTCAGCGTCCG	TGGGCGATCCATTGATGTC	50.0	52.6	59.1	57.6	175 bp	1.0	68.3
ycf1_p4	TTTCAGCGTCCGGTTGATC	TGGGCGATCCATTGATGTC	52.6	52.6	58.2	57.6	167 bp	1.0	67.5
ycf1_p5	ACTTGAGTTGTTTCAGCGTC	TGGGCGATCCATTGATGTC	45.0	52.6	56.6	57.6	177 bp	1.0	65.6