Hotspot

ndhA

SSC · 116015-118092 · 2078 bp

Back to Athyrium

Pi 0.0155

MarkerSeek score 55.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2078
variable_sites: 96
pi: 0.0155
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTGAAACTGAAGTGAAGGAGA	TATGGGAGAGGTTGATCGCT	40.9	50.0	57.0	57.9	2121-2128 bp	1.0	58.9
ndhA_p2	TGAACTGAAACTGAAGTGAAGGA	TATGGGAGAGGTTGATCGCT	39.1	50.0	57.9	57.9	2124-2131 bp	1.0	58.3
ndhA_p3	AACTGAAACTGAAGTGAAGGAGA	TATGGGAGAGGTTGATCGCT	39.1	50.0	57.6	57.9	2122-2129 bp	1.0	56.2
ndhA_p4	ACTGAAACTGAAGTGAAGGAGA	AAAGGAATGAAGCTCGCAGA	40.9	45.0	57.0	57.5	2174-2181 bp	1.0	55.7
ndhA_p5	TGAACTGAAACTGAAGTGAAGGA	AAAGGAATGAAGCTCGCAGA	39.1	45.0	57.9	57.5	2177-2184 bp	1.0	55.2