Hotspot

rps12-rrn16

IRa · 138927-140857 · 1931 bp

Back to Athyrium

Pi 0.00948

MarkerSeek score 64.20

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: IRa
length_bp: 1931
variable_sites: 66
pi: 0.00948
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	AGCCGTTACCTCAAACTTGG	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	2055-2096 bp	1.0	82.4
rps12-rrn16_p2	GCCGTTACCTCAAACTTGGA	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	2054-2095 bp	1.0	82.4
rps12-rrn16_p3	GGTTAATCGAACTCTCGCGA	TCCGACTTGCATGTGTTAGG	50.0	50.0	57.8	57.9	2034-2075 bp	1.0	81.8
rps12-rrn16_p4	AGGTTAATCGAACTCTCGCG	TCCGACTTGCATGTGTTAGG	50.0	50.0	57.8	57.9	2035-2076 bp	1.0	81.8
rps12-rrn16_p5	TCGAACTCTCGCGACTTTAC	TCCGACTTGCATGTGTTAGG	50.0	50.0	57.8	57.9	2028-2069 bp	1.0	81.4