Hotspot

ycf1-chlN

SSC · 125059-125434 · 376 bp

Back to Athyrium

Pi 0.043
MarkerSeek score 65.00
Primer available yes
Label ycf1-chlN

Feature Metadata

label_name
ycf1-chlN
feature_type
igs
region
SSC
length_bp
376
variable_sites
46
pi
0.043
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-chlN_p1 CGATTTCAGCCCAGTTAGTGA ACAGACGGTAGATGTCTAA 47.6 42.1 58.0 52.1 440-449 bp 1.0 48.7
ycf1-chlN_p2 TCAGCCCAGTTAGTGACAAAG ACAGACGGTAGATGTCTAA 47.6 42.1 57.9 52.1 435-444 bp 1.0 48.7
ycf1-chlN_p3 CAGCCCAGTTAGTGACAAAGT ACAGACGGTAGATGTCTAA 47.6 42.1 58.1 52.1 434-443 bp 1.0 48.7
ycf1-chlN_p4 TCAGCCCAGTTAGTGACAAA ACAGACGGTAGATGTCTAA 45.0 42.1 56.7 52.1 435-444 bp 1.0 48.7
ycf1-chlN_p5 TTCAGCCCAGTTAGTGACAA ACAGACGGTAGATGTCTAA 45.0 42.1 56.7 52.1 436-445 bp 1.0 48.7