Hotspot

ccsA-ndhD

SSC · 111606-112006 · 401 bp

Back to Athyrium

Pi 0.0324

MarkerSeek score 65.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 41
pi: 0.0324
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCACCCCCAAATTCAAGGAA	ATTTTGGTCCGAGGGAGGTA	50.0	50.0	58.0	58.0	883-895 bp	1.0	85.0
ccsA-ndhD_p2	CCACCCCCAAATTCAAGGAA	TTTGGTCCGAGGGAGGTATT	50.0	50.0	58.0	58.0	881-893 bp	1.0	85.0
ccsA-ndhD_p3	CCACCCCCAAATTCAAGGAA	TTTTGGTCCGAGGGAGGTAT	50.0	50.0	58.0	58.0	882-894 bp	1.0	85.0
ccsA-ndhD_p4	CCACCCCCAAATTCAAGGAA	AACGGAGCCATATCGCAAAT	50.0	45.0	58.0	58.0	1265-1277 bp	1.0	84.3
ccsA-ndhD_p5	CCACCCCCAAATTCAAGGAA	TTTGTAGCCATCGGGATTGG	50.0	50.0	58.0	57.9	1307-1319 bp	1.0	84.1