Hotspot

rpl32-trnP-GGG

SSC · 109267-109951 · 685 bp

Back to Athyrium

Pi 0.0339

MarkerSeek score 70.90

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 685
variable_sites: 79
pi: 0.0339
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	CGGCGGTAAAGACCTAAACA	GAGGTCACAGGTTCGAATCC	50.0	55.0	57.9	58.0	1089-1102 bp	1.0	88.4
rpl32-trnP-GGG_p2	CGGCGGTAAAGACCTAAACA	GGAGGTCACAGGTTCGAATC	50.0	55.0	57.9	58.0	1090-1103 bp	1.0	88.4
rpl32-trnP-GGG_p3	CGGCGGTAAAGACCTAAACA	CTGGCACTGCTTCCTAAGAG	50.0	55.0	57.9	58.0	1454-1488 bp	1.0	88.1
rpl32-trnP-GGG_p4	CGGCGGTAAAGACCTAAACA	CACAAACGCGAAGAGGGATA	50.0	50.0	57.9	58.0	1688-1722 bp	1.0	87.5
rpl32-trnP-GGG_p5	CGGCGGTAAAGACCTAAACA	CACCATAGCGGCTTTTTGTG	50.0	50.0	57.9	58.0	1422 bp	0.230769	48.5