Hotspot

rrn16-rps12

IRb · 92923-94853 · 1931 bp

Back to Athyrium

Pi 0.00948

MarkerSeek score 64.20

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: IRb
length_bp: 1931
variable_sites: 66
pi: 0.00948
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	TCCGACTTGCATGTGTTAGG	AGCCGTTACCTCAAACTTGG	50.0	50.0	57.9	58.1	2055-2096 bp	1.0	82.4
rrn16-rps12_p2	TCCGACTTGCATGTGTTAGG	GCCGTTACCTCAAACTTGGA	50.0	50.0	57.9	58.1	2054-2095 bp	1.0	82.4
rrn16-rps12_p3	TCCGACTTGCATGTGTTAGG	GGTTAATCGAACTCTCGCGA	50.0	50.0	57.9	57.8	2034-2075 bp	1.0	81.8
rrn16-rps12_p4	TCCGACTTGCATGTGTTAGG	AGGTTAATCGAACTCTCGCG	50.0	50.0	57.9	57.8	2035-2076 bp	1.0	81.8
rrn16-rps12_p5	TCCGACTTGCATGTGTTAGG	TCGAACTCTCGCGACTTTAC	50.0	50.0	57.9	57.8	2028-2069 bp	1.0	81.4