Hotspot

rpl23-trnI-CAU

LSC · 82190-82327 · 138 bp

Back to Athyrium

Pi 0.0561
MarkerSeek score 70.70
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
LSC
length_bp
138
variable_sites
24
pi
0.0561
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 TGTTTCCCCAAATCGCAGAA TTTGACACGGTTTTGCCAAC 45.0 45.0 58.0 58.0 722-732 bp 0.692308 74.4
rpl23-trnI-CAU_p2 TTGTTTCCCCAAATCGCAGA TTTGACACGGTTTTGCCAAC 45.0 45.0 58.0 58.0 723-733 bp 0.692308 74.4
rpl23-trnI-CAU_p3 TGTTTCCCCAAATCGCAGAA TGGCGAATTCACAGGTTCAA 45.0 45.0 58.0 58.0 619-629 bp 0.692308 73.5
rpl23-trnI-CAU_p4 TGTTTCCCCAAATCGCAGAA TTGGCGAATTCACAGGTTCA 45.0 45.0 58.0 58.0 620-630 bp 0.692308 73.5
rpl23-trnI-CAU_p5 TTGTTTCCCCAAATCGCAGA TGGCGAATTCACAGGTTCAA 45.0 45.0 58.0 58.0 620-630 bp 0.692308 73.5