Hotspot

trnP-UGG-psaJ

LSC · 62929-63293 · 365 bp

Back to Athyrium

Pi 0.0398

MarkerSeek score 68.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 365
variable_sites: 44
pi: 0.0398
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CCCAAAACAAACACGCTACC	AGCCAAACCATATTGCAGCT	50.0	45.0	57.9	58.1	458-476 bp	1.0	86.6
trnP-UGG-psaJ_p2	CCCAAAACAAACACGCTACC	CAGGGGCCGTAGATAGGTAT	50.0	55.0	57.9	57.8	434-452 bp	1.0	86.2
trnP-UGG-psaJ_p3	CCAAAACAAACACGCTACCG	AGCCAAACCATATTGCAGCT	50.0	45.0	58.3	58.1	457-475 bp	1.0	85.6
trnP-UGG-psaJ_p4	CCAAAACAAACACGCTACCG	CAGGGGCCGTAGATAGGTAT	50.0	55.0	58.3	57.8	433-451 bp	1.0	85.2
trnP-UGG-psaJ_p5	CCCAAAACAAACACGCTACC	GCAAGCCAAACCATATTGCA	50.0	45.0	57.9	57.6	461-479 bp	1.0	84.6