Hotspot

petA-psbJ

LSC · 59613-60443 · 831 bp

Back to Athyrium

Pi 0.0351

MarkerSeek score 72.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 86
pi: 0.0351
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAAGTCCAGTTGGCAG	GCCGGTACACTTGTGATAGG	50.0	55.0	57.7	58.1	941-954 bp	0.923077	82.5
petA-psbJ_p2	TGAGAAAGTCCAGTTGGCAG	TTGGGGTCGTCTCCTCAATA	50.0	50.0	57.7	57.8	884-897 bp	0.923077	81.5
petA-psbJ_p3	TGAGAAAGTCCAGTTGGCAG	TGGGGTCGTCTCCTCAATAA	50.0	50.0	57.7	57.8	883-896 bp	0.923077	81.4
petA-psbJ_p4	TGAGAAAGTCCAGTTGGCAG	CTGTAGCCGGTACACTTGTG	50.0	55.0	57.7	58.3	946-959 bp	0.923077	80.6
petA-psbJ_p5	TGAGAAAGTCCAGTTGGCAG	GGTAGGTACTGTAGCCGGTA	50.0	55.0	57.7	57.7	954-967 bp	0.923077	80.1