Hotspot

trnC-GCA-trnG-GCC

LSC · 29790-30236 · 447 bp

Back to Athyrium

Pi 0.0379
MarkerSeek score 81.00
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
LSC
length_bp
447
variable_sites
50
pi
0.0379
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 GATTTGCAGTCCTCCGTCTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.9 58.2 475-519 bp 1.0 81.6
trnC-GCA-trnG-GCC_p2 GATTTGCAGTCCTCCGTCTT GGGTTCGATTCCCGCTATC 50.0 57.9 57.9 57.8 477-521 bp 1.0 81.4
trnC-GCA-trnG-GCC_p3 GATTTGCAGTCCTCCGTCTT GGTTCGATTCCCGCTATCC 50.0 57.9 57.9 57.8 476-520 bp 1.0 81.4
trnC-GCA-trnG-GCC_p4 TCCGTCTTACCGCTTGACTA GTTCGATTCCCGCTATCCG 50.0 57.9 58.2 58.2 463-507 bp 1.0 81.1
trnC-GCA-trnG-GCC_p5 TCCGTCTTACCGCTTGACTA GGTTCGATTCCCGCTATCC 50.0 57.9 58.2 57.8 464-508 bp 1.0 80.9