Hotspot

trnS-GCU-ycf12

LSC · 7968-8525 · 558 bp

Back to Athyrium

Pi 0.0275

MarkerSeek score 64.90

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 57
pi: 0.0275
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	GGGAGAGAGAGGGATTCGAA	GCAAGTTGCGCAATTATTTCT	55.0	38.1	57.9	56.4	675-679 bp	1.0	65.3
trnS-GCU-ycf12_p2	AAGGGAGAGAGAGGGATTCG	GCAAGTTGCGCAATTATTTCT	55.0	38.1	57.9	56.4	677-681 bp	1.0	65.2
trnS-GCU-ycf12_p3	AGCAATCCGACGCTTTAGAC	GCAAGTTGCGCAATTATTTCT	50.0	38.1	58.4	56.4	624-628 bp	1.0	63.6
trnS-GCU-ycf12_p4	GGGAGAGAGAGGGATTCGAA	GCAAGTTGCGCAATTATTTC	55.0	40.0	57.9	54.9	675-679 bp	1.0	61.1
trnS-GCU-ycf12_p5	AAGGGAGAGAGAGGGATTCG	GCAAGTTGCGCAATTATTTC	55.0	40.0	57.9	54.9	677-681 bp	1.0	61.1