Hotspot

psbK-psbI

LSC · 7161-7692 · 532 bp

Back to Athyrium

Pi 0.0287

MarkerSeek score 71.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 532
variable_sites: 51
pi: 0.0287
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCAGTTAGTTTTCG	TCCGGGATCGTTTGACAAAA	50.0	45.0	58.0	57.7	646-650 bp	1.0	85.0
psbK-psbI_p2	CAAGCCGCAGTTAGTTTTCG	ATCTAATCCCTACGGCCAGG	50.0	55.0	58.0	58.4	672-676 bp	1.0	83.9
psbK-psbI_p3	AGCCGCAGTTAGTTTTCGAT	TCCGGGATCGTTTGACAAAA	45.0	45.0	57.9	57.7	644-647 bp	0.923077	80.3
psbK-psbI_p4	AAGCCGCAGTTAGTTTTCGA	TCCGGGATCGTTTGACAAAA	45.0	45.0	58.1	57.7	645-648 bp	0.923077	80.0
psbK-psbI_p5	AGCCGCAGTTAGTTTTCGAT	ATCTAATCCCTACGGCCAGG	45.0	55.0	57.9	58.4	670-673 bp	0.923077	79.3