Hotspot

matK-rps16

LSC · 2958-3596 · 639 bp

Back to Athyrium

Pi 0.0232

MarkerSeek score 64.10

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 49
pi: 0.0232
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GAAGGGATCCAGAAGTTGTCT	GCCTGAACAAGTCGGAATCA	47.6	50.0	57.0	58.2	700-716 bp	1.0	69.7
matK-rps16_p2	GAAGGGATCCAGAAGTTGTCT	TGCCTGAACAAGTCGGAATC	47.6	50.0	57.0	58.2	701-717 bp	1.0	69.6
matK-rps16_p3	AAGGGATCCAGAAGTTGTCT	GCCTGAACAAGTCGGAATCA	45.0	50.0	55.7	58.2	699-715 bp	1.0	67.5
matK-rps16_p4	AGGGATCCAGAAGTTGTCTT	GCCTGAACAAGTCGGAATCA	45.0	50.0	55.7	58.2	698-714 bp	1.0	67.5
matK-rps16_p5	AGGGATCCAGAAGTTGTCTT	TGCCTGAACAAGTCGGAATC	45.0	50.0	55.7	58.2	699-715 bp	1.0	67.5