Hotspot

ycf1

Genome · 65963-74572 · 8610 bp

Back to Athrotaxis

Pi 0.0122

MarkerSeek score 58.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 8610
variable_sites: 99
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCACTCGGTCTTCAAGCTC	TTCTGTTCACGTGTGGAAGG	55.0	50.0	58.0	58.1	734-761 bp	1.0	84.9
ycf1_p2	CTCTCACTCGGTCTTCAAGC	TTCTGTTCACGTGTGGAAGG	55.0	50.0	58.0	58.1	736-763 bp	1.0	84.8
ycf1_p3	GCTCTCACTCGGTCTTCAAG	TTCTGTTCACGTGTGGAAGG	55.0	50.0	58.0	58.1	737-764 bp	1.0	84.8
ycf1_p4	CTCACTCGGTCTTCAAGCTC	AGTCTTCCTTCAGGGTCCTC	55.0	55.0	58.0	58.1	579-597 bp	1.0	84.7
ycf1_p5	CTCACTCGGTCTTCAAGCTC	GTCTTCCTTCAGGGTCCTCT	55.0	55.0	58.0	58.1	578-596 bp	1.0	84.7