Hotspot

trnG-UCC

Genome · 43514-43536 · 23 bp

Back to Athrotaxis

Pi 0.0435

MarkerSeek score 77.50

Primer available yes

Label trnG-UCC

Feature Metadata

label_name: trnG-UCC
feature_type: tRNA
region: Genome
length_bp: 23
variable_sites: 1
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	CCAGGGTATTCATAGGAGAGGT	GGACAAAAGATCGATCTGTATGC	50.0	43.5	58.1	57.7	177 bp	1.0	40.5
trnG-UCC_p2	ACCAGGGTATTCATAGGAGAGG	GGACAAAAGATCGATCTGTATGC	50.0	43.5	58.1	57.7	178 bp	1.0	40.4
trnG-UCC_p3	ACCAGGGTATTCATAGGAGAGG	TGGACAAAAGATCGATCTGT	50.0	40.0	58.1	54.4	179 bp	1.0	40.4
trnG-UCC_p4	CCAGGGTATTCATAGGAGAGGT	TGGACAAAAGATCGATCTGT	50.0	40.0	58.1	54.4	178 bp	1.0	40.4
trnG-UCC_p5	CCAGGGTATTCATAGGAGAGGT	TTGGACAAAAGATCGATCTGT	50.0	38.1	58.1	55.2	179 bp	1.0	40.4