Hotspot

infA

Genome · 92920-93228 · 309 bp

Back to Athrotaxis

Pi 0.055

MarkerSeek score 67.20

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: Genome
length_bp: 309
variable_sites: 17
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ATTTTGCGAACAGAAGCACG	AAACAGTTCGAGTAGCTGCT	45.0	45.0	58.0	57.2	904-1040 bp	1.0	81.3
infA_p2	ACGAATCAGACGGCACTTTT	AAACAGTTCGAGTAGCTGCT	45.0	45.0	58.1	57.2	929-1065 bp	1.0	80.5
infA_p3	GCACCTTTGAATCCACAAGC	AAACAGTTCGAGTAGCTGCT	50.0	45.0	57.9	57.2	1333-1450 bp	1.0	79.8
infA_p4	GTTTGCCCTCGTGTATCTGT	AAACAGTTCGAGTAGCTGCT	50.0	45.0	57.9	57.2	1291-1408 bp	1.0	79.7
infA_p5	TTGCGAAAACTTGCTCGAAC	AAACAGTTCGAGTAGCTGCT	45.0	45.0	57.9	57.2	1255-1372 bp	1.0	79.7