Hotspot

trnV-GAC-LK118_pgr004

Genome · 113791-114640 · 850 bp

Back to Athrotaxis

Pi 0.0231
MarkerSeek score 65.80
Primer available yes
Label trnV-GAC-LK118_pgr004

Feature Metadata

label_name
trnV-GAC-LK118_pgr004
feature_type
igs
region
Genome
length_bp
850
variable_sites
19
pi
0.0231
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-LK118_pgr004_p1 ACCTTGACGTGGTGAAAGTC TCCCAAGGGCAGGTTACTTA 50.0 50.0 58.1 58.0 979-1000 bp 1.0 83.1
trnV-GAC-LK118_pgr004_p2 CCTTGACGTGGTGAAAGTCA TCCCAAGGGCAGGTTACTTA 50.0 50.0 58.1 58.0 978-999 bp 1.0 83.1
trnV-GAC-LK118_pgr004_p3 ACCTTGACGTGGTGAAAGTC CCCAAGGGCAGGTTACTTAC 50.0 55.0 58.1 57.9 978-999 bp 1.0 82.5
trnV-GAC-LK118_pgr004_p4 CCTTGACGTGGTGAAAGTCA CCCAAGGGCAGGTTACTTAC 50.0 55.0 58.1 57.9 977-998 bp 1.0 82.5
trnV-GAC-LK118_pgr004_p5 ACCTTGACGTGGTGAAAGTC GGGCAGGTTACTTACGCATT 50.0 50.0 58.1 58.3 973-994 bp 1.0 81.5