Hotspot

rpl22-rps19

Genome · 97143-97195 · 53 bp

Back to Athrotaxis

Pi 0.113

MarkerSeek score 72.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: Genome
length_bp: 53
variable_sites: 6
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	AACCACGAAGTTGATGAGCT	TTCCAGGGACATGCGAAAAA	45.0	45.0	57.4	58.0	219-224 bp	1.0	78.0
rpl22-rps19_p2	AACCACGAAGTTGATGAGCT	TTTCCAGGGACATGCGAAAA	45.0	45.0	57.4	58.0	220-225 bp	1.0	78.0
rpl22-rps19_p3	AACCACGAAGTTGATGAGCT	TTTTCCAGGGACATGCGAAA	45.0	45.0	57.4	58.0	221-226 bp	1.0	78.0
rpl22-rps19_p4	AACCACGAAGTTGATGAGCT	TCCATTTTCCAGGGACATGC	45.0	50.0	57.4	58.1	225-230 bp	1.0	76.9
rpl22-rps19_p5	AACCACGAAGTTGATGAGCT	ATTTTCCAGGGACATGCGAA	45.0	45.0	57.4	57.8	222-227 bp	1.0	76.4