Hotspot

ycf1-rps12

Genome · 74573-75626 · 1054 bp

Back to Athrotaxis

Pi 0.0254

MarkerSeek score 69.20

Primer available yes

Label ycf1-rps12

Feature Metadata

label_name: ycf1-rps12
feature_type: igs
region: Genome
length_bp: 1054
variable_sites: 25
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps12_p1	ACATCTGTTTAGCCAAGGCA	CCTTTACGCTGAGGACATCC	45.0	55.0	57.4	58.1	1131-1188 bp	1.0	80.6
ycf1-rps12_p2	ACATCTGTTTAGCCAAGGCA	GCACATACACCCTAGCACAT	45.0	50.0	57.4	57.7	1154-1211 bp	1.0	78.4
ycf1-rps12_p3	ACATCTGTTTAGCCAAGGCA	TTTCTATTGGCTGTCTCGCG	45.0	50.0	57.4	58.4	1085-1142 bp	1.0	78.2
ycf1-rps12_p4	ACATCTGTTTAGCCAAGGCA	ACTCCTTTACGCTGAGGACA	45.0	50.0	57.4	58.4	1134-1191 bp	1.0	78.1
ycf1-rps12_p5	ACATCTGTTTAGCCAAGGCA	GCTGTCTCGCGTTTCTAGTA	45.0	50.0	57.4	57.5	1076-1133 bp	1.0	76.8