Hotspot

rpl32-trnM-CAU

Genome · 55853-56383 · 531 bp

Back to Athrotaxis

Pi 0.042

MarkerSeek score 67.90

Primer available yes

Label rpl32-trnM-CAU

Feature Metadata

label_name: rpl32-trnM-CAU
feature_type: igs
region: Genome
length_bp: 531
variable_sites: 22
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnM-CAU_p1	ATCTTCGATGTCGCAGCAAT	ACCAGCTGTATCGCTATCCT	45.0	50.0	58.1	58.0	1274-1276 bp	1.0	82.9
rpl32-trnM-CAU_p2	GATAACGATCGGCAAGCTCA	ACCAGCTGTATCGCTATCCT	50.0	50.0	58.1	58.0	1152-1154 bp	1.0	82.1
rpl32-trnM-CAU_p3	ATCTTCGATGTCGCAGCAAT	CCGTCTGTAGCACTCATAGC	45.0	55.0	58.1	57.9	1357-1359 bp	1.0	81.7
rpl32-trnM-CAU_p4	TCCCACTCCTCCCTTTCTTT	ACCAGCTGTATCGCTATCCT	50.0	50.0	57.9	58.0	1458 bp	0.5	58.9
rpl32-trnM-CAU_p5	TTCCCACTCCTCCCTTTCTT	ACCAGCTGTATCGCTATCCT	50.0	50.0	57.9	58.0	1459 bp	0.5	58.9