Hotspot

chlB-trnQ-UUG

Genome · 7824-8015 · 192 bp

Back to Athrotaxis

Pi 0.0209

MarkerSeek score 65.00

Primer available yes

Label chlB-trnQ-UUG

Feature Metadata

label_name: chlB-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 192
variable_sites: 4
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnQ-UUG_p1	TCCTAGAGGAGCGTGCATAA	TGGTCCTGTTATTGCGAAGG	50.0	50.0	57.9	57.9	336-337 bp	1.0	79.8
chlB-trnQ-UUG_p2	TCCTAGAGGAGCGTGCATAA	GGTCCTGTTATTGCGAAGGT	50.0	50.0	57.9	57.9	335-336 bp	1.0	79.8
chlB-trnQ-UUG_p3	GAGCAGGACCAGCATACATC	TGGTCCTGTTATTGCGAAGG	55.0	50.0	58.1	57.9	268-269 bp	1.0	79.6
chlB-trnQ-UUG_p4	GAGCAGGACCAGCATACATC	GGTCCTGTTATTGCGAAGGT	55.0	50.0	58.1	57.9	267-268 bp	1.0	79.6
chlB-trnQ-UUG_p5	AGCTACTCGTAGGGTTCCAA	TGGTCCTGTTATTGCGAAGG	50.0	50.0	57.8	57.9	291-292 bp	1.0	78.7