Hotspot

rpl32-trnL-UAG

SSC · 117811-118539 · 729 bp

Back to Atalantia

Pi 0.0212

MarkerSeek score 66.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 729
variable_sites: 41
pi: 0.0212
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCGAAATCCCTTGCTACTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	746-841 bp	1.0	88.0
rpl32-trnL-UAG_p2	AGCGAAATCCCTTGCTACTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	747-842 bp	1.0	88.0
rpl32-trnL-UAG_p3	AGCGAAATCCCTTGCTACTG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	756-851 bp	1.0	87.0
rpl32-trnL-UAG_p4	AGCGAAATCCCTTGCTACTG	GCTGCATAGCCCAATACCAT	50.0	50.0	58.0	58.1	1351-1440 bp	1.0	86.9
rpl32-trnL-UAG_p5	AGCGAAATCCCTTGCTACTG	CTTGGTGCCGTTATATCGCT	50.0	50.0	58.0	58.1	1219-1293 bp	0.375	58.3